材料表征方法理论与实践课程的改革与建设*

通过与超星专业慕课制作团队合作搭建网络教学平台,将线上慕课教学与线下实验教学相结合,对材料表征方法理论与实践课程进行了全方位的改革。与传统实验教学相比,改革后的课程不仅能够激发学生的学习兴趣,实现时间空间的相对自由,还可以完善考核机制,提升教学质量,从而为高校化学研究生实验课程的改革提供一定的借鉴意义。     

Isometric artifacts from polymerase chain reaction-massively parallel sequencing analysis of short tandem repeat loci: An emerging issue from a new technology?

The recent introduction of polymerase chain reaction (PCR)-massively parallel sequencing (MPS) technologies in forensics has changed the approach to allelic short tandem repeat (STR) typing because sequencing cloned PCR fragments enables alleles with identical molecular weights to be distinguished based on their nucleotide sequences. Therefore, because PCR fidelity mainly depends on template integrity, new technical issues could arise in the interpretation of the results obtained from the degraded samples. In this work, a set of DNA samples degraded in vitro was used to investigate whether PCR-MPS could generate “isometric drop-ins” (IDIs; i.e., molecular products having the same length as the original allele but with a different nucleotide sequence within the repeated units). The Precision ID GlobalFiler NGS STR panel kit was used to analyze 0.5 and 1 ng of mock samples in duplicate tests (for a total of 16 PCR-MPS analyses). As expected, several well-known PCR artifacts (such as allelic dropout, stutters above the threshold) were scored; 95 IDIs with an average occurrence of 5.9 IDIs per test (min: 1, max: 11) were scored as well. In total, IDIs represented one of the most frequent artifacts. The coverage of these IDIs reached up to 981 reads (median: 239 reads), and the ratios with the coverage of the original allele ranged from 0.069 to 7.285 (median: 0.221). In addition, approximately 5.2% of the IDIs showed coverage higher than that of the original allele. Molecular analysis of these artifacts showed that they were generated in 96.8% of cases through a single nucleotide change event, with the C > T transition being the most frequent (85.7%). Thus, in a forensic evaluation of evidence, IDIs may represent an actual issue, particularly when DNA mixtures need to be interpreted because they could mislead the operator regarding the number of contributors. Overall, the molecular features of the IDIs described in this work, as well as the performance of duplicate tests, may be useful tools for managing this new class of artifacts otherwise not detected by capillary electrophoresis technology.     

CTA在急性复杂性大咯血患者行介入栓塞术前的应用价值分析

本文探讨了CT血管造影(CT angiography,CTA)在急性复杂性大咯血患者行介入栓塞术前的应用价值。回顾性选择49例急性复杂性大咯血患者CTA影像资料,通过与术中数字减影血管造影(digital subtraction angiography,DSA)结果进行对比,发现CTA对罪犯血管诊断准确率为89.33%,对多动脉交通吻合诊断准确率为86.96%。Kappa检验CTA与DSA检查结果一致性较高,受试者特征曲线(ROC)分析CTA诊断急性复杂性大咯血罪犯血管的曲线下面积(AUC)为0.947(95%CI:0.903~0.990,P=0.000),灵敏度89.33%,特异度100.00%。通过随访发现CTA指导下行介入栓塞术治疗有效率91.84%,术后1年无大咯血复发病例。     

Validity of the equivalent-photon approximation for the production of massive spin-1 particles

We point out that the equivalent-photon approximation (EPA) for processes with massive spin-1 particles in the final state would have validity in a more restricted kinematic domain than for processes where it is commonly applied, viz., those with spin-1/2 or spin-0 particles in the final state. We obtain the criterion for the validity ofEPA for the two-photon production of a pair of charged, massive, point-like spin-1 particlesV ^±, each of massM and with a standard magnetic moment (κ=1). In a process in which one of the photons is real and the other virtual with four-momentumq, the condition for the validity ofEPA is |q ²|≪M ², in addition to the usual condition |q ²|≪W ²,W being theV ⁺ V ⁻ invariant mass. In a process in which both photons are virtual (with four-momentaq andq′), our condition is |q ²||q′²|W ⁴ ≪ 16M ⁸, in addition to |q ²| ≪M ², |q′²| ≪M ² and |q ²| ≪W ², |q′²| ≪W ². Even when these extra conditions permitting the use ofEPA are not fulfilled, convenient approximate expressions may still be obtained assuming merely |q ²| ≪W ² and |q′²| ≪W ². We also discuss how the extra conditions are altered when the vector bosons are incorporated in a spontaneously broken gauge theory. Examples ofW boson production in Weinberg-Salam model are considered for which the condition |q ²||q′²|W ⁴ ≪ 16M ⁸ is shown to be removed.     

Rate analysis of ZF receiver for uplink cell-free massive MIMO systems with D2D communications

This paper proposes a transmission structure of zero forcing (ZF) receiver for uplink cell-free massive multiple-input multiple-output (MIMO) systems with device-to-device (D2D) communications, followed by a rate analysis. We assumed that D2D users (DUEs) can utilize orthogonal radio resources to improve the efficiency of the scarce utilization or repurpose the time–frequency-spectrum resources currently used by the cell-free users (CFUEs). Assuming that the imperfect channel state information (CSI) is realizable, after that, the use-and-forget bounding technique is then used to respectively obtain the closed-form expressions of the CFUEs and DUEs, which provide the lower bounds on the ergodic approximate realizable rate of both communication links. First, we calculate the minimum-mean-square error (MMSE) estimation for all channels. Then, the derived results of the achievable uplink sum rate provide us with a tool that enables us to explain how some important parameters, such as the number of access points (APs)/CFUEs, each AP/CFUE/antenna, and the density of DUEs, affect system performance, highlighting the significance of cooperation between cell-free massive MIMO and D2D communication.     

Systematic benchmarking of large molecular dynamics simulations employing GROMACS on massive multiprocessing facilities

The influence of the total number of cores, the number of cores dedicated to Particle mesh Ewald (PME) calculation and the choice of single vs. double precision on the performance of molecular dynamic (MD) simulations in the size of 70,000 to 1.7 million of atoms was analyzed on three different high‐performance computing facilities employing GROMACS 4 by running about 6000 benchmark simulations. Small and medium sized systems scaled linear up to 64 and 128 cores, respectively. Systems with half a million to 1.2 million atoms scaled linear up to 256 cores. The best performance was achieved by dedicating 25% of the total number of cores to PME calculation. Double precision calculations lowered the performance by 30–50%. A database for collecting information about MD simulations and the achieved performance was created and is freely available online and allows the fast estimation of the performance that can be expected in similar environments. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011     

非重子冷暗物质粒子的研究进展

暗物质问题目前已受到物理学界的高度关注，因为这一课题的研究和进展，将直接影响到粒子物理、天体物理和宇宙学的发展方向。当前世界上已有一些大的实验组正在开展这方面的研究工作，并已取得了一定的结果；文章重点介绍两个重要的探测实验，即中意合作DAMA组（Dark Matter Group)100kg Nal（Tl）探测器阵列实验和美国的CDMS（Cold Dark Matter Search）实验组的低温探测器实验。详细介绍了DAMA实验的物理分析方法及其实验结果，并同CDMS实验结果进行了相应的比较。     

海量数据导入与导出MATLAB的有效方法

本文结合实例介绍了几种将海量统计数据快速、准确地导入与导出MATLAB系统的方法。克服了以往在编程窗口直接键入原始数据进行处理的诸多问题,因此对保证统计分析结果的准确性与加速MATLAB在统计分析领域的应用有着很重要的意义。     

大质量恒星的形成:理论与观测

文章简要叙述了有关大质量恒星形成的理论以及相关观测证据。目前大质量恒星形成的理论主要有两种，即吸积说和并合说．吸积说认为，大质量星可能与小质量星形成于相似的过程；并合说主张大质量星可能是由小质量年轻星碰撞合并而成．解决这两种理论争论的关键在于在大质量星附近能否观测到吸积盘的存在，最新的观测表明大质量星更有可能是通过吸积增加自身的质量，但最终解决这一问题可能还需要更多的观测证据。文章还提出了一些本领域尚未解决的问题，为感兴趣的研究者提供参考。